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alcian blue stain kit  (Vector Laboratories)


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    Vector Laboratories alcian blue stain kit
    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Alcian Blue Stain Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alcian+blue+(ph+2%2E5)+stain+kit/Alcian+Blue+(pH+2%2E5)+Stain+Kit/pmc13114135-135-19-27
    Average 95 stars, based on 95 article reviews
    alcian blue stain kit - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis"

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

    Journal: Gut Microbes

    doi: 10.1080/19490976.2026.2662638

    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Figure Legend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Techniques Used: Staining, Western Blot, Quantitative RT-PCR, Expressing

    Related Articles

    Staining:

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis
    Article Snippet: Stained sections were imaged using VS120 Virtual Slide Microscope (Olympus) through the OlyVIA software. .. Alcian blue staining was performed to visualize acidic mucopolysaccharides and glycosaminoglycans in goblet cells of colon sections using the Alcian Blue Stain Kit (pH 2.5, Cat# H-3501, Vector Laboratories), following the manufacturer’s instructions. ..

    Article Title: NADPH-producing enzymes restrict the formation of pancreatic precancerous lesions.
    Article Snippet: H&E staining was performed using Mayer’s haematoxylin solution and Eosin Y (Thermo Fisher Scientific). .. Blue and Nuclear Fast Red staining was performed using the Alcian Blue (pH 2.5) Stain Kit (Vector Laboratories, H-3501) per the manufacturer’s protocol. .. Imaging was performed automatically using a whole-slide scanner (Vectra Polaris, Akoya Biosciences) or manually using an Olympus BX53F microscope.

    Article Title: DPY30 is an epigenetic decoupler linking replication stress to immunoediting in pancreatic cancer
    Article Snippet: .. For Alcian Blue (Vector Laboratories, H3501) and 464 Masson’s Trichrome staining (VitroViewTM Masson’s Trichrome Stain Kit, VB-3016), 465 samples were processed according to the manufacturers’ protocols. ..

    Article Title: Weaning drives microbiome-mediated epigenetic regulation to shape immune memory in mice
    Article Snippet: IHC was performed as described previously using primary antibodies against Ki-67 (Thermo Fisher, MA5-14520, 1:100) and CD3 (Abcam, ab16669, 1:100). .. Alcian blue (AB) (pH 2.5) staining (Vector Labs, H-3501) was conducted following manufacturer instructions. ..

    Article Title: NADPH-producing enzymes restrict the formation of pancreatic precancerous lesions
    Article Snippet: H&E staining was performed using Mayer’s haematoxylin solution and Eosin Y (Thermo Fisher Scientific). .. Blue and Nuclear Fast Red staining was performed using the Alcian Blue (pH 2.5) Stain Kit (Vector Laboratories, H-3501) per the manufacturer’s protocol. .. Imaging was performed automatically using a whole-slide scanner (Vectra Polaris, Akoya Biosciences) or manually using an Olympus BX53F microscope.

    Article Title: Chemical Pancreatectomy Alleviates Tumor Burden in a Novel Inducible Mouse Model of Intraductal Papillary Mucinous Neoplasm
    Article Snippet: .. Alcian blue (pH 2.5; Vector Laboratories, H-3501) and Masson's trichrome (Abcam, ab150686) staining were performed per kit instructions. .. For immunofluorescence, antigen retrieval was performed with heat-mediated citrate buffer (pH 6.0), followed by 1-hour serum blocking and overnight incubation at 4°C with primary antibodies: rabbit anti-Sox9 (1:200; Thermo Fisher, PA5-23383); mouse anti-insulin (1:200; Thermo Fisher, 53-9769-82); rabbit anti-amylase (1:200; Cell Signaling Technology, 37T96T); rabbit anti-CK19 (1:200; Abcam, ab133496).



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    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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    alcian blue ab  (Vector Laboratories)
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    ( a ) Representative images of <t>IHC</t> <t>staining</t> for Ki-67 and <t>Alcian</t> blue (AB) in colon tissues from mice under SPF (n = 4) or GF (n = 3) housing conditions. Scale bars, 50 μm. ( b ) Quantification of Ki-67 + cells and AB + goblet cells across intestinal segments (duodenum, jejunum, ileum, and colon) in SPF and GF mice. All data are presented as mean ± s.e.m. of at least 3 biological replicates. P values were calculated using unpaired (two-tailed) t -test.
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    Image Search Results


    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Gut Microbes

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

    doi: 10.1080/19490976.2026.2662638

    Figure Lengend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: Alcian blue staining was performed to visualize acidic mucopolysaccharides and glycosaminoglycans in goblet cells of colon sections using the Alcian Blue Stain Kit (pH 2.5, Cat# H-3501, Vector Laboratories), following the manufacturer’s instructions.

    Techniques: Staining, Western Blot, Quantitative RT-PCR, Expressing

    ( a ) Representative images of IHC staining for Ki-67 and Alcian blue (AB) in colon tissues from mice under SPF (n = 4) or GF (n = 3) housing conditions. Scale bars, 50 μm. ( b ) Quantification of Ki-67 + cells and AB + goblet cells across intestinal segments (duodenum, jejunum, ileum, and colon) in SPF and GF mice. All data are presented as mean ± s.e.m. of at least 3 biological replicates. P values were calculated using unpaired (two-tailed) t -test.

    Journal: Nature Microbiology

    Article Title: Weaning drives microbiome-mediated epigenetic regulation to shape immune memory in mice

    doi: 10.1038/s41564-026-02295-6

    Figure Lengend Snippet: ( a ) Representative images of IHC staining for Ki-67 and Alcian blue (AB) in colon tissues from mice under SPF (n = 4) or GF (n = 3) housing conditions. Scale bars, 50 μm. ( b ) Quantification of Ki-67 + cells and AB + goblet cells across intestinal segments (duodenum, jejunum, ileum, and colon) in SPF and GF mice. All data are presented as mean ± s.e.m. of at least 3 biological replicates. P values were calculated using unpaired (two-tailed) t -test.

    Article Snippet: Alcian blue (AB) (pH 2.5) staining (Vector Labs, H-3501) was conducted following manufacturer instructions.

    Techniques: Immunohistochemistry, Two Tailed Test